Table 1.

Beetle genomes and transcriptomes included in the study. Taxonomic classifications are listed with data sources, as well as completeness (Benchmarking Universal Single-Copy Ortholog, BUSCO, score, C = complete, S = complete single-copy, D = complete duplicated, F = fragmented, M = missing), number of predicted proteins, and number of orthologous groups (OGs) with genes from each species. The outgroup species used in the phylogeny, Stylops melittae, belongs to the order Strepsiptera, which is the sister group of Coleoptera [33]

Species	Short form	Suborder	Family	Type of assembly	Accession	Bioproject	Source	BUSCO score (1658 genes)	Predicted proteins	OrthoDB groups (OG)
Cicindela hybrida	CHYBR	Adephaga	Carabidae	Transcriptome	GDMH01000000	PRJNA286505	1KITE, this study	C: 90.4 [S: 84.9%, D: 5.5%], F: 3.4%, M: 6.2%	13,916	8111
Calosoma frigidum	CFRIG	Adephaga	Carabidae	Transcriptome	GDLF01000000	PRJNA286499	1KITE, this study	C: 78.3% [S: 73.5%, D: 4.8%], F: 9.8%, M: 11.9%	9844	6742
Elaphrus aureus	EAURE	Adephaga	Carabidae	Transcriptome	GDPI01000000	PRJNA286520	1KITE, this study	C: 92.4% [S: 85.9%, D: 6.5%], F:2.2%, M: 5.4%	12,808	8020
Noterus clavicornis	NCLAV	Adephaga	Noteridae	Transcriptome	GDNA01000000	PRJNA286561	1KITE, Vasilikopoulos et al. [34]	C: 90.9% [S: 84.1%, D: 6.8%], F: 4.4%, M: 4.7%	12,981	7918
Haliplus fluviatilis	HFLUV	Adephaga	Haliplidae	Transcriptome	GDMW01000000	PRJNA286525	1KITE, Vasilikopoulos et al. [34]	C: 91.8% [S: 76.3%, D: 15.5%], F: 4.3%, M: 3.9%	19,408	8528
Cybister lateralimarginalis	CLATE	Adephaga	Dytiscidae	Transcriptome	GDLH01000000	PRJNA286512	1KITE, Vasilikopoulos et al. [34]	C: 88.2% [S: 83.1%, D: 5.1%], F: 3.7%, M:8.1%	13,916	7256
Sinaspidytes wrasei	SWRAS	Adephaga	Aspidytidae	Transcriptome	GDNH01000000	PRJNA286492	1KITE, Vasilikopoulos et al. [34]	C: 87.0% [S: 76.7%, D: 10.3%], F: 4.0%, M: 9.0%	13,392	7721
Dineutus sp.	DINEU	Adephaga	Gyrinidae	Transcriptome	GDNB01000000	PRJNA286516	1KITE, Vasilikopoulos et al. [34]	C: 71.9% [S: 51.6%, D: 20.3%], F: 13.6%, M: 14.5%	14,644	7089
Gyrinus marinus	GMARI	Adephaga	Gyrinidae	Transcriptome	GAUY01000000	PRJNA219564	1KITE, Misof et al. [35]	C: 81.5% [S: 79.0%, D: 2.5%], F: 8.1%, M: 10.4%	13,867	7663
Aleochara curtula	ACURT	Polyphaga	Staphylinidae	Transcriptome	GATW01000000	PRJNA219522	1KITE, Misof et al. [35]	C: 91.4% [S: 87.5%, D: 3.9%], F: 4.0%, M: 4.6%	20,280	8513
Anoplophora glabripennis	AGLAB	Polyphaga	Cerambycidae	Genome	GCF_000390285	PRJNA167479	I5k, McKenna et al. [21]	C: 96.9% [S: 95.8%, D: 1.1%], F: 2.7%, M: 0.4%	22,035	10,959
Agrilus planipennis	APLAN	Polyphaga	Buprestidae	Genome	GCF_000699045	PRJNA230921	I5k, unpublished	C: 92.5% [S: 91.2%, D: 1.3%], F: 4.5%, M: 3.0%	15,497	9089
Dendroctonus ponderosae	DPOND	Polyphaga	Curculionidae	Genome	GCF_000355655	PRJNA162621	Keeling et al. [36]	C: 91.2% [S: 86.0%, D: 5.2%], F: 4.1%, M: 4.7%	13,457	8518
Leptinotarsa decemlineata	LDECE	Polyphaga	Chrysomelidae	Genome	GCF_000500325	PRJNA171749	I5k, Schoville et al. [37]	C: 88.9% [S: 87.5%, D: 1.4%], F: 9.9%, M: 1.2%	24,671	11,149
Laparocerus tessellatus	LTESS	Polyphaga	Curculionidae	Transcriptome	10.5281/zenodo.1336288	N/A	This study, Seppey et al. [38]	C: 93.8% [S: 91.9%, D: 1.9%], F: 1.4%, M: 4.8%	18,448	8616
Meloe violaceus	MVIOL	Polyphaga	Meloidae	Transcriptome	GATA01000000	PRNJA219578	1KITE, Misof et al. [35]	C: 90.3% [S: 85.6%, D: 4.7%], F: 5.9%, M: 3.8%	14,295	8480
Onthophagus taurus	OTAUR	Polyphaga	Scarabaeidae	Genome	GCF_000648695	PRJNA167478	I5k, unpublished	C: 96.2% [S: 93.9%, D: 2.3%], F: 2.5%, M: 1.3%	17,483	9315
Tribolium castaneum	TCAST	Polyphaga	Tenebrionidae	Genome	GCF_000002335	PRJNA12540	Richards et al. [39]	C: 97.0% [S: 96.5%, D: 0.5%], F: 1.6%, M:1.4%	16,645	9429
Stylops melittae	SMELI	Outgroup	Stylopidae	Transcriptome	GAZM02000000	PRNJA219603	1KITE, Misof et al. [35]	C: 76.5% [S: 55.0%, D: 21.5%], F: 7.1%, M: 16.4%	13,026	6104