Fig. 3.

BMX-ARHGAP overexpression elevates the BMX-SH2 protein expression via ARHGAP 5′UTR. a Protein expression of BXM, ARHGAP and BMX-ARHGAP in transfected SGC7901 cells, as measured by western blot assay; b BMX exon 10–11 and ARHGAP12 5′UTR mRNA expression after transfection, as determined by means of RT-qPCR; c protein expression of BMX-SH2 after transfection, as measured by western blot assay; *p < 0.05 vs. the cells transfected with pcDNA3.1 or sh-NC. Measurement data were expressed as mean ± standard deviation and analyzed by one-way analysis of variance; each experiment was repeated three times. BMX bone marrow kinase on chromosome X, ARHGAP RhoA GTPase activating protein, RT-qPCR reverse transcription quantitative polymerase chain reaction