Table 1.
List of antibodies and conditions used in this work
| Antibody | Dilution (WB/IF) | Clone and/or reference | Supplier | |
|---|---|---|---|---|
| 53BP1 | Rabbit polyclonal | -; 1/200 | H300, sc-22,760 | Santa Cruz Biotechnology |
| 53BP1 | Rabbit polyclonal | -; 1/200 | NB100–304 | Novus Biologicals |
| Histone H4-K16 ac | Rabbit monoclonal | 1/500; 1/400 | ab109463 | Abcam |
| Nbs1 | Mouse monoclonal | -; 1/200 | 611,871 | BD Biosciences |
| VRK1 | Mouse monoclonal | 1/1000; 1/200 | 1B5 | Own production [74] |
| VRK1 | Rabbit polyclonal | 1/1000;- | VC | Own production [74] |
| VRK1 (N-term) | Rabbit polyclonal | -; 1/200 | HPA000660 | Sigma-Aldrich |
| β-actin | Mouse monoclonal | 1/2000; − | AC15/A5441 | Sigma-Aldrich |
| γH2AX | Mouse monoclonal | -; 1/200 | Clone JBW301; 05–636 | Millipore |
| Anti-mouse IgG (WB) | Goat Anti-Mouse IgG, DyLight 680 (red) | 1/10000; − | 35,518 | Thermo Scientific |
| Anti-rabbit IgG (WB) | Goat Anti-Rabbit IgG, DyLight 800 (green) | 1/10000; − | 35,571 | Thermo Scientific |
| Goat anti-Mouse IgG (IF) | Goat anti-Mouse IgG linked to Cy3 (red) | -; 1/1000 | 115–165-146 | Jackson ImmunoResearch; West Grove, PA, USA |
| Goat anti-rabbit IgG (IF) | Goat anti-rabbit IgG linked to Cy2 (green) | -; 1/1000 | 111–225-144 | Jackson ImmunoResearch; West Grove, PA, USA |