Table 3.
NNMT expression reduces the chemo-sensitivities of human breast cancer cells to ADM and PTX
| IC50 of ADM (μM) | IC50 of PTX (nM) | |||
|---|---|---|---|---|
| Cell lines | Mean ± SEM | Fold change | Mean ± SEM | Fold change |
| SK-BR-3/Vector | 0.12 ± 0.02 | 12.32 ± 1.20 | ||
| SK-BR-3/NNMT-1 | 0.40 ± 0.05** | 3.33 | 17.41 ± 1.95 | 1.41 |
| SK-BR-3/NNMT-2 | 1.00 ± 0.11** | 8.33 | 24.50 ± 2.83* | 1.99 |
| MCF7/Vector | 0.40 ± 0.05 | 20.12 ± 1.93 | ||
| MCF7/NNMT-1 | 0.82 ± 0.09* | 2.05 | 28.35 ± 2.14* | 1.41 |
| MCF7/NNMT-2 | 0.92 ± 0.11* | 2.30 | 31.18 ± 3.05* | 1.55 |
| MDA-MB-231/NC | 1.40 ± 0.09 | 12.40 ± 1.13 | ||
| MDA-MB-231/NNMT shRNA 1# | 0.72 ± 0.06** | 0.51 | 7.23 ± 0.78* | 0.58 |
| MDA-MB-231/NNMT shRNA 2# | 0.61 ± 0.05** | 0.44 | 6.20 ± 0.74* | 0.49 |
Data represent the mean ± SEM of three independent experiments. Statistical significance was detected between the NNMT overexpression or downregulation groups and each matched the control group (SK-BR-3/Vector, MCF/vector or MDA-MB-231/NC)
*p < 0.05, **p < 0.01