Table 4.

Inhibition of SIRT1 expression enhances the chemo-sensitivities to ADM and PTX in breast cancer cells overexpressing NNMT

	ADM (μM)		PTX (nM)
Cell lines	Mean ± SEM	Fold change	Mean ± SEM	Fold change
SK-BR-3/NNMT-2	1.02 ± 0.09		25.63 ± 2.06
SK-BR-3/NNMT-2+EX527	0.88 ± 0.08	0.86	21.10 ± 1.67	0.82
SK-BR-3/NNMT-2+SIRT1 siRNA	0.49 ± 0.06**	0.48	17.37 ± 1.56*	0.68
MCF7/NNMT-2	0.95 ± 0.08		31.92 ± 2.49
MCF7/NNMT-2+EX527	0.53 ± 0.06*	0.56	25.15 ± 1.53	0.79
MCF7/NNMT-2+SIRT1 siRNA	0.35 ± 0.05**	0.37	21.95 ± 1.39*	0.69
MDA-MB-231	1.23 ± 0.09		12.40 ± 1.13
MDA-MB-231+EX527	0.92 ± 0.11	0.75	7.20 ± 0.78*	0.58
MDA-MB-231+SIRT1 siRNA	0.64 ± 0.05**	0.52	6.10 ± 0.74**	0.49

Data represent the mean ± SEM of three independent experiments. Statistical significance was detected between the EX527, SIRT1 siRNA groups, and control groups treated only with DMSO

*p < 0.05, **p < 0.01