Fig. 6.

HDAC3 inhibition affects the miR-200a/Keap1/Nrf2 pathway and associated protective effects in endothelial monolayer permeability. a Quantification of miR-200a expression in db/+ and db/db mice with/without RGFP966 treatment, measured by real-time quantitative PCR. b–e Quantifications of HO-1, CAT, IL-1β, and IL-6 mRNA expression in db/+ and db/db mice with or without RGFP966 treatment, measured by real-time quantitative PCR. Data are expressed as mean ± SEM. *P < 0.05 vs. db/+, ^#P < 0.05 vs. db/db, n = 4 mice per group. f Relative transendothelial permeability (Dextran Extravasation) of cultured HBMEC, induced by HG-IL1β insult and treated with RGFP966 or Nrf2 inhibitor trigonelline. g TEER measurement of HBMEC monolayers induced by HG-IL1β insult and treated with RGFP966 or Nrf2 inhibitor trigonelline. Data are expressed as mean ± SEM. *P < 0.05 vs. norm, ^#P < 0.05 vs. HG-IL1β and P < 0.05 vs. HG-IL1β+RGFP966, n = 4 cultures per group