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. 2019 May 17;12:243. doi: 10.1186/s13071-019-3500-1

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Expression, purification and western blotting of recombinant TcAQP1c peptide. a SDS-PAGE analysis of the prokaryotic expression of recombinant TcAQP1c. Lane M: protein molecular weight maker; Lane 1: supernatant expression of induced empty pET32a; Lane 2: insoluble expression of induced empty pET32a; Lane 3: supernatant expression of non-induced recombinant pET32a plasmids; Lane 4: insoluble expression of non-induced recombinant pET32a plasmids; Lane 5: supernatant expression of induced recombinant pET32a plasmids; Lane 6: insoluble expression of induced recombinant pET32a plasmids. b Purification of recombinant TcAQP1c peptide. Lane M: protein molecular weight maker; Lanes 1–5: elution using 20 mM, 50 mM, 100 mM, 250 mM or 500 mM of imidazole, respectively. c Western blot analysis of recombinant TcAQP1c peptide. Lane M: protein molecular weight maker; Lane 1: polyclonal antibody against the recombinant TcAQP1c peptide; Lane 2: pre-immune serum