Fig. 8.

ERβ-ligand treatment during the remyelination phase of the cuprizone model further enhances remyelination and increases mature oligodendrocytes and OPCs. (A–D) Representative images of MBP⁺ stained myelin (A), GSTπ⁺ mature oligodendrocytes (B), CC1⁺ immature/mature oligodendrocytes (C), and NG2⁺ OPCs (D) in the corpus callosum (CC) of WT mice. (Scale bars, 100 μm.) (E–H) Quantitative analysis of MBP mean intensity (E) and percentage of GSTπ⁺ mature oligodendrocytes (F), CC1⁺ immature/mature oligodendrocytes (G), and NG2⁺ OPCs (H) showed that ERβ ligand-treated 9w+3w/ERβ WT mice had increased myelin, mature oligodendrocytes, and OPCs compared with vehicle-treated 9w+3w/Veh WT mice, and ERβ-ligand treatment-mediated increases were not observed in Olig1 CKO mice (9w+3w/Veh CKO vs. 9w+3w/ERβ CKO; *P < 0.05, **P < 0.01, and ****P < 0.0001).