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. 2019 Apr 29;116(20):10140–10149. doi: 10.1073/pnas.1901787116

Fig. 7.

Fig. 7.

Effect of microglia depletion on AAV8-sCX3CL1 cone rescue. (A) Representative flow cytometry gating of microglia (CD11b+ Ly6G/Ly6C−) and CD11b− Ly6G/Ly6C+ populations in P50 rd1 retinas with or without PLX3397 treatment from P20–P49. Panels are gated on live cells (DAPI−) following doublet exclusion. (B) Fraction of microglia and CD11b− Ly6G/Ly6C+ cells remaining relative to controls in P50 rd1 retinas infected at P0–P1 with AAV8-GFP alone or AAV8-GFP plus AAV8-sCX3CL1 after 30 d of PLX3397 treatment. Retinas from littermates without PLX3397 treatment were used as controls. (C) Flat-mounted P50 rd1 retinas from PLX3397-treated mice infected with AAV8-GFP or AAV8-GFP plus AAV8-sCX3CL1. (Scale bars: 1 mm.) (D) Quantification of cone survival in the central retina of P50 rd1 retinas from PLX3397-treated mice infected with AAV8-GFP or AAV8-GFP plus AAV8-sCX3CL1. Data are shown as mean ± SEM [n = 3–4 animals per condition (A), n = 9–18 animals per condition (D)]. ****P < 0.0001 by two-tailed Student’s t test. N.S., not significant.