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. 2019 Apr 29;116(20):9953–9958. doi: 10.1073/pnas.1818573116

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Fig. 1. — Construction of various DAF-MCP chimeras. (A) Diagrammatic representation of DAF, MCP, and the DAF-MCP chimeras. The domains are numbered and the boundaries are marked by the vertical lines. The numbers associated with the vertical lines represent the boundary residues and are according to UniProt numbering. The linkers connecting the DAF domains are in red, while the linkers connecting the MCP domains are in blue; the same color scheme is used to depict the linkers in the DAF-MCP chimeras. D2M2–4 (ML) represents the chimera with the M1–M2 interdomain linker. (B, Upper) SDS/PAGE analysis of DAF, MCP, and the DAF-MCP chimeras expressed in Pichia. (B, Lower) SDS/PAGE analysis of DAF, DAF mutant D2D3, and the DAF-MCP chimeras D2D3M3M4 and D2D3D4M4 expressed in E. coli. MW, molecular weight.