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Substitution of the putative factor I interaction sites in the D3 domain of D2D3M3M4 generates a molecule with CP- and AP-DAA and C3b- and C4b-CFA. (A) C3b-CFA and (B) C4b-CFA of MCP and the D2D3M3M4 substitution mutants multi-4 and multi-5. (C) AP-DAA and (D) CP-DAA of DAF, D2D3M3M4, and its substitution mutants multi-4 and multi-5. “Inh.” denotes inhibitor concentration.
