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. 2019 Apr 26;116(20):9941–9946. doi: 10.1073/pnas.1901183116

Fig. 2.

Fig. 2.

C-terminal domains of PARP-1 are responsible for PARP-1 targeting to the TSS of the hsp70 locus, whereas DNA-binding domains target PARP-1 to areas outside of the promoter region. (A–D) The comparison of recombinant protein distribution within the hsp70 locus, ChIP assay before and after HS: PARP-1-YFP (A); ZIII-A-W-Cat-YFP (B); W-Cat-YFP (C); and ZI-ZII-YFP (D). (E) PARP-1 isoforms with C-terminal domains rescue transcription activation at the hsp70 locus following HS in parp-1C03256 mutants. The level of hsp70 mRNA was recorded before and after 30 min of HS treatment using quantitative RT PCR. (F) PARP-1 isoforms with C-terminal domains rescue histone H3 displacement from hsp70 locus, following HS in parp-1C03256 mutants. ChIP assay compares amounts of H3 histone in the promoter region of the hsp70 locus in wild-type and parp-1C03256 mutant animals expressing full-length PARP-1 (PARP-1-YFP) or PARP-1 deletional isoforms (ZI-ZII, ZIII-A-W-Cat, W-Cat) before (−) and after (+) 30 min of HS treatment. All error bars are based on the average of triplicates.