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. 2019 May 1;116(20):9877–9882. doi: 10.1073/pnas.1819400116

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Fig. 4. — Identification of TEAD4 depalmitoylating enzymes. (A) Analysis of palmitoylation by Acyl-RAC analysis. Depalmitoylation of TEAD4 by the overexpression of serine hydrolases was determined by Acyl-RAC. (B) Increase of TEAD palmitoylation by the depletion of APT2. Endogenous serine hydrolases in MCF-10A cells were depleted by siRNA transfection. The palmitoylation status of TEAD1 and TEAD4 was determined by Acyl-RAC. (C) Quantitative analysis of serine hydrolases mRNA expression. The expression of serine hydrolases in MCF-10A cells at low or high densities was determined by qPCR.