Figure 7.

Relevance of NOD2 and RIP2 in the activation of signaling pathways in macrophages stimulated with heat-killed Aa. Macrophages differentiated from the bone marrow of WT, NOD2-KO or RIP2-KO mice were stimulated with heat-killed Aa for 10, 30 and 60 min. Total cell lysates were harvested and 30 μg used in multi-ligand ELISAs to detect phosphorylated forms of p65, p38, JNK and STAT3. These results were further normalized by the expression of total p65. Data were analyzed as relative change (fold change) to the normalized expression of each target protein by vehicle (PBS)-stimulated macrophages from WT mice in each experimental period (10, 30 and 60 min). Bars indicate averages and SDs of duplicate measurements. Data obtained using a pool of cell lysates from three independent experiments using cells derived from 6–8 different animals of each genotype.