Fig. 6.

Overexpression of miR-30a-5p inhibits tumor growth by targeting CD73 and increases the sensitivity of pancreatic cancer to gemcitabine. a The miR-30a-5p was confirmed significantly increased in the subcutaneous tumors after the intratumoral injection of miR-30a-5p agomir by FISH. b miR-30a-5p agomir or control oligo mixture was injected into the xenografts in a multi-site injection manner every 3 days for 2 weeks, and tumor growth was measured in mice by bioluminescence imaging 30 days post-implant (n = 4 in each group). c Tumor growth curves in mice at the indicated days. d The expression level of CD73 and TNFR2 in the tumor tissues from miR-30a-5p agomir or negative control–treated nude mice was detected by IHC. e, f Analysis of the changes in miRNA and mRNA expression levels in gemcitabine-resistant pancreatic cancer cell line PANC-1 based on the GEO databases GSE80616 and GSE80617. g PANC-1 and CFPAC-1 were stimulated with different concentrations of gemcitabine (nM) for 48 h. The expression of miR-30a-5p was detected by PCR. h, i PANC-1 and CFPAC-1 were stimulated with different concentrations of gemcitabine (nM) for 48 h. The expression of CD73 was detected by PCR and western blot. j The IC₅₀ value to gemcitabine decreased in miR-30a-5p-overexpressed cells compared to negative control. k The IC₅₀ value to gemcitabine decreased in CD73 knockdown cells compared to negative control. l CD73 overexpression inhibited the effect of miR-30a-5p on promoting the cell-killing effect of gemcitabine. Data are expressed as mean ± SEM (n = 3). *p < 0.05