Fig. 2.

Visualization of intestinal CD8⁺DCs. (A) To confirm the presence of intestinal CD8⁺ DCs, total leukocytes from rainbow trout intestine were fixed and stained with anti-CD8α (green) and anti-MHC II (red) mAbs, counterstained with DAPI (blue) and analyzed by fluorescence microscopy. Scale bars, 5 μm. (B) In order to study the location of CD8⁺ DCs within the tissue, cryostat sections were prepared from rainbow trout intestine, fixed and labelled with anti-CD8α (green) and anti-MHC class II (red) Abs, counterstained with DAPI (blue), and analyzed by fluorescence microscopy. A representative image is shown (upper panels, scale bars, 20 μm), together with magnifications of an area containing a CD8⁺ DC (lower panels, scale bars 20 μm and 5 μm). LP = lamina propria; Lu = lumen; CT = Cytotoxic CD8⁺ T cell; DC = CD8⁺ MHC II⁺ dendritic cell. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)