Fig. 3.

Transcription of mammalian cross-presenting markers and toll-like receptor (TLR) genes by rainbow trout intestinal CD8⁺ DCs. Intestinal CD8⁺ DCs were isolated by flow cytometry, total RNA extracted and used to study the levels of transcription of cross-presenting DCs markers (A) and TLRs (B) by real time PCR. RNA was also obtained from RTS-11 monocytes-macrophages for comparative reasons. Results are shown as relative expression levels to the endogenous control EF1α (mean + SD; n = 3–9).