Fig. 4.

Transcriptional response of intestinal CD8⁺ DCs to poly I:C. Intestinal leukocytes in L-15 medium supplemented with 5% FCS were incubated with 50 μg/ml poly I:C for 16 h at 20 °C. Control cells incubated with media alone were also included. After this time, CD8⁺ DCs were FACS isolated from poly I:C-treated and control cultures. Total RNA was extracted from the FACS isolated cells and used to determine the levels of transcription of different immune genes by real time PCR. Data are shown as the gene expression relative to the expression of endogenous control EF-1α (mean + SD; n = 4). Asterisks denote statistical differences between values obtained in poly I:C-treated cells and cells from control cultures. Statistical analyses were performed, where * p ≤ 0.05 and **p ≤ 0.01.