Fig. 6.

Phagocytic capacity of intestinal CD8⁺DCs. Intestinal leukocytes were incubated with Crimson Red fluorescent polystyrene beads (1 μm diameter) at a ratio 1:10 (cell/beads) for 16 h and then centrifuged through a 3% BSA 4.5% glucose gradient in order to remove non-ingested beads. Cells were then labelled with anti-CD8α and analyzed by flow cytometry. (A) Representative plots showing gated lymphoid cells (upper panels) and myeloid cell (lower panels) are shown. CD8⁻ and CD8⁺ cells were further selected to analyze the fluorescence of internalized beads (shown in the histograms). The percentage of cells containing beads were determined using control samples without beads to set the positive threshold. Mean percentages of phagocytic cells (B) and median fluorescence intensity of the beads (C) in each subpopulation are shown as mean + SD (n = 12). The phagocytic capacity of intestinal CD8⁺ DCs was compared to that of gills CD8⁺ DCs, studying both the percentage of phagocytic cells (D) and MFI of beads (E). (n = 12). Statistical analyses were performed and asterisks denote significant differences between groups as indicated. **p ≤ 0.01, ***p ≤ 0.005. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)