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Expression of kidney markers (SIX2, PAX8, Nephrin, and Podocin), endothelial marker (CD31), mesenchymal markers (PDGFR-β and Vimentin), and pluripotency-associated markers (TRA-1-81, NANOG, and SSEA4) in 3D-NPCs. Cell nuclei were stained with Hoechst. Zoom-in pictures show the subcellular localization of the respective proteins. Pictures were taken under 20x magnification. The scale bars represent a length of 50 μm.
