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. 2019 May 5;2019:5387850. doi: 10.1155/2019/5387850

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Copyright © 2019 Gabriel Romero Liguori et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Influence of micrometer-sized topography and TGF-β1 stimulation on mesenchymal gene expression in adhered ASC over seven days. Expression of ACTA2 (a) and TAGLN (b) increased over 3 days, independent of TGF-β1 stimulation and topography (flat vs. 1 or 11 μm wavelength). For the net influence of TGF-β1 and topography, the area under the curve was determined for total expression of ACTA1 (c) and TAGLN (d), respectively. This showed no differences between flat material and topographies, irrespective of TGF-β1. Values represent mean ± SEM of 3 independent experiments. Statistical analysis was performed comparing the flat topography to both of the linear topography patterns for both TGF-β1-stimulated and not stimulated groups.