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. 2019 May 2;2019:7896524. doi: 10.1155/2019/7896524

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Copyright © 2019 Shiki Fujino and Norikatsu Miyoshi.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Analysis of Oct4-EGFP-positive cells. (a) Representative FACS of enriched Oct4-EGFP cells by sorting. Oct4-positive cells and Oct4-negative cells were shown. Oct4-high cells (green area) and Oct4-negative cells (pink area) were analyzed. (b) Oct4 mRNA expression of Oct4-high cells (green area of (a)) was high compared with that of Oct4-negative cells (pink area of (a); n = 3, P < 0.05). (c) Expressions of CD44 and CD133 were high in Oct4-high cells (green area of (a)) compared with Oct4-negative cells (pink area of (a)). (d) Gene set enrichment analysis (GSEA) of Oct4-high cells (green area of (a)) and Oct4-negative cells (pink area of (a)). Representative GSEA was shown, and genes relating to WNT protein binding (P < 0.001) and fibroblast growth factor receptor binding (P < 0.001) were enriched in Oct4-high cells.