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. 2019 May 2;2019:1378639. doi: 10.1155/2019/1378639

Figure 6.

Figure 6

ANTXR1/TEM8-positive PDAC cells possess CSC functional and molecular traits. (a) Sphere-forming capacity of ANTXR1+ and ANTXR− cells sorted from PancB023, Panc253, and Panc354 cells following seven days in non-adherent culture conditions. Shown is the total number of spheres determined/1 ml categorized by size in μM (triplicate samples and n = 2 experiments). (b) RTqPCR analysis of relative mRNA expression levels for the indicated genes in ANTXR1+ and ANTXR1− cells sorted from PancB023. mRNA expression levels for each target gene are normalized to β-actin levels. Fold changes were calculated compared to ANTXR1− (set at 1.0) (triplicate samples and n = 2 experiments).