Figure 5.

Induction of osteogenic gene expression under standard static cell culture condition conditions. qPCR analysis of osteogenic markers (BGLAP, ALPL, SPP1, RUNX2, COL1A1, and SPARC) was performed on L88/5 cells stimulated with the osteogenic cocktail (20 nM 1α,25-dihydroxyvitamin D3, 50 μM L-ascorbic acid 2-phosphate, and 10 mM β-glycerophosphate) up to 10 days. B2M and GAPDH were used as reference genes (2^-ΔΔCT method). Fold changes from control untreated cells at day 0 were calculated. All data are reported as mean ± SEM of four biological replicates. ^∗p < 0.05 and ^∗∗p < 0.01.