Figure 2.

Inhibitory effects of TJ001 on lipid accumulation in DU145 cells. Cells were incubated for 48 h with or without TJ001 (200 μg/mL). (a) The content of ATP was measured using a commercial kit (Promega, USA). Data are presented as the mean ± SD (∗P < 0.05 compared with the control). We analyzed (b) the expression of lipid metabolism-related proteins, (c) the effects of compound C (c.c) on phosphorylated AMPK (p-AMPK). (d) Lipid accumulation was visualized using an Olympus CKX41 inverted microscope at ×300 magnification [left panel; Oil Red O stained cells with 0 μg/mL, right panel; with 200 μg/mL TJ001].