Figure 3.

TJ001 induces G1/S phase arrest in DU145 cells. Cell cycle distribution of DU145 cells measured by flow cytometry. (a) Cells were incubated with TJ001 (200 μg/mL) treatment for 48 h. All floating and attached cells were harvested and fixed in 95% ethanol. The stained cells with PI were performed to examine cell cycle progression (b) The graphs showed a cell cycle distribution in DU145 prostate cancer cells. Data represents mean ± SD (∗∗ p< 0.01) (c) CKI (Cyclin kinase inhibitor) and cyclin proteins, involved with G1/S phase arrest, were performed. (d) Total and phosphorylated forms of protein synthesis-related proteins in mTOR signaling pathway were detected using immunoblotting assay.