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. 2019 May 5;2019:2460353. doi: 10.1155/2019/2460353

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Copyright © 2019 Sooyeon Kang et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effects of mutant p53 knockdown with TJ001 treatment in DU145 cells. After cells were transfected with p53 siRNA or control siRNA, and then cells were treated with TJ001. (a) The cell viability was evaluated by WST-1 assay. (∗P<0.05, ∗∗P<0.01, and ∗∗∗P<0.001). The total lysates were assessed by western blot analyses. Following to transfection, (b) expression of p-AMPK/AMPK ratio, (c) levels of cell cycle-related proteins expression and (d) PARP, capsase-3, capsase-8, and capsase-9 were measured. (e) Quantitative analysis of caspase-3 activity in vitro. Cell proteins were diluted in Cell Lysis Buffer and assayed for caspase-3 activity using DEVD-pNA as substrate. Results were measured at 405 nm wavelength (∗∗∗p<0.001).