Figure 5.

Berberine attenuates the Aβ_1-42-induced reactive oxygen species generation and malondialdehyde elevation in primary cultured hippocampal neurons. (a) Representative images of DCF staining in hippocampal neurons for the indicated treatment. Scale bar = 250 μm. (b) Quantification of DCF intensities in (a). (c) MDA were significantly elevated in Aβ_1-42-treated neurons compared to vehicle-treated neurons and partially restored in berberine-treated neurons in a dose-dependent manner. Values are expressed as the mean ± standard error of the mean of 4 independent experiments. ^∗∗p < 0.01 and ^∗∗∗p < 0.001 vs. vehicle-treated group, ^#p < 0.05 and ^##p < 0.01 vs. the Aβ-treated group. Aβ: cells were treated with Aβ_1-42 at 0.5 μM.