Table 5.

TCS genotoxicity and carcinogenicity.

Model	Effect	Classification
HepG2 cells	Global DNA hypomethylation	Limited evidence of carcinogenicity
V79 cells	Chromosomal aberrations
Mouse	Somatic mutation (positive spot test) Increased incidence of liver tumors Aggravated hepatocellular carcinoma Exacerbated colon tumorigenesis
D. polymorpha	DNA damage (positive comet assay)	N/A
U. tumidus	DNA strand breaks (Hoescht 33342 fluorescence)
E. Fetida	DNA damage (positive Comet assay)
D. magna	DNA damage (positive Comet assay)
A. salina	DNA damage (positive Comet assay)
H. fossilis	DNA damage (positive Comet assay)
C. auratus	DNA damage (positive Comet assay)
O. mykiss	DNA damage (positive Comet assay)
T. thermophila	DNA damage (positive Comet assay)
A. cepa	Chromosomal stickiness, reduced mitotic activity, and ana-telophase bridges (positive Feulgen reaction)

N/A = data from non-mammalian animals are not considered for ECHA mutagenicity/carcinogenicity classification.