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Effects of serum on human CD34+ cell transduction in xenograft mice. (a) Human CD34+ cell transduction was performed using a 24h prestimulation with cytokines and an additional 24h of fresh media containing the same cytokines and viral vector addition. (b) Human CD34+ cells were transduced with serum-free X-VIVO10 media or DMEM with 10% FBS, and transplanted into our xenograft mouse model.
