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. 2019 May 18;16:104. doi: 10.1186/s12974-019-1488-2

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© The Author(s). 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Effects of HS on AMPK activation. a BV-2 cells treated with different concentration of HS for 2 h. b BV-2 cells treated with 1 μM HS for different times. c BV-2 cells treated with 1 μM HS with/without 500 ng/mL LPS for 2 or 4 h. d BV-2 cells treated with 1 μM HS with/without 500 ng/mL LPS or 1 μM Compound C (CC) for 2 h. Levels of p-AMPK, AMPK and β-actin were determined with use of Western blot. e Representative immunoblots of protein levels for phosphorylated AMPK (p-AMPK), AMPK and β-actin in ipsilateral cortex at 3 days following HI insult. N = 4/group. Values represent the mean ± SD, *p < 0.05, **p < 0.01 according to ANOVA