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. 2019 May 18;16:104. doi: 10.1186/s12974-019-1488-2

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© The Author(s). 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Effects of HS on HI-induced microglial activation. a Representative Iba-1 staining within the core of infarcted cortex at 3 days following HI insult. N = 4/group. b Representative flow cytometric lots of CD11b⁺/CD45^high cells within ipsilateral cortex at 3 days following HI insult. N = 4/group. Right histograms of average percentage of CNS-associated phagocytes (CD11b⁺/CD45^high cells) in live single immune cells from brains. c Representative brain images as obtained at 72 h following HI. Arrows indicate sites of significant edema. Brain water content was determined at 72 h following HI insult, N = 6/group. d Representative samples stained with TTC. Quantified infarct volume is indicated by the white area. N = 6/group. Values represent the mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001 according to ANOVA