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. 2019 Apr 13;9(9):2606–2617. doi: 10.7150/thno.32344

Figure 1.

Figure 1

Targeting PRMT5 activity reduces the self-renewal capacity of HCC cells. (A) Real-time PCR analysis of the expression levels of epigenetic enzymes. (B) The expression of stemness markers was decreased in Huh-7 and Hep3B cells treated with DW14800. (C) DW14800 reduced the frequency of primary spheroid formation of Huh-7 and Hep3B cells. (D) Representative photographs of primary tumor spheres. Scale bars = 100 μm. (E) Quantification analysis of the size of primary tumor spheres formed from HCC cells. (F) The frequency of secondary spheroid formation of HCC cells. (G) Representative photographs of secondary tumor spheres. Scale bars = 100 μm. (H) Quantification analysis of the size of secondary tumor spheres formed. The data are presented as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001.