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. 2019 Apr 13;9(9):2678–2693. doi: 10.7150/thno.31884

Figure 2.

Figure 2

S. elongatus PCC 7942 enhances angiogenesis in vivo and in vitro. (A) Gross view of wounds treated with PBS, S. elongatus-light and S. elongatus-dark at day 12 post-wounding from the undersurface. Scale bar: 2 mm. (B) Representative images of vascular marker CD31-stained wound sections at day 12 post-wounding. Scale bar: 50 μm. (C) Quantification of the number of CD31-positive vessels. n = 3 per group. (D) CCK-8 analysis of proliferation of human microvascular endothelial cells (HMECs). n = 4 per group. (E-F) Representative images of transwell migration assay in HMECs (E) and quantification of the migrated cells (F). Scale bar: 100 μm. n = 3 per group. (G-H) Representative images of wound healing assay in HMECs (G) and quantification of the migration rates (H). Scale bar: 200 μm. n = 3 per group. (I) Representative images of HMECs' tube formation. Scale bar: 200 μm. (J) Quantification of the total tube length, total branching points and total loops. n = 3 per group. Data are plotted as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.