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. 2019 Apr 13;9(9):2678–2693. doi: 10.7150/thno.31884

Figure 3.

Figure 3

EVs secretion is required for the pro-angiogenic effects of S. elongatus PCC 7942. (A) Total protein contents of S. elongatus-EVs. n = 3 per group. (B) CCK-8 analysis of HMECs' proliferation. n = 4 per group. S: S. elongatus PCC 7942. (C-D) Representative images of transwell migration assay in HMECs (C) and quantitative analysis of the migrated cells (D). Scale bar: 100 μm. n = 3 per group. (E-F) Representative images (E) and quantification (F) of wound healing assay for HMECs. Scale bar: 200 μm. n = 3 per group. (G-H) Representative images (G) and quantification (H) of HMECs' tube formation. Scale bar: 200 μm. n = 3 per group. Data are plotted as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.