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. 2019 Apr 13;9(9):2460–2474. doi: 10.7150/thno.31097

Figure 4.

Figure 4

HCP5 knockdown inhibits cell proliferation and metastasis in nude mice. (A) Mice were subcutaneously injected with cells stably silenced for HCP5 (shHCP5) or control cells (shCtrl). Images of the tumor lumps from the indicated groups at the endpoint of the experiment. n = 6 mice per group. (B) Tumor formation in mice treated as in (A) was monitored at the indicated time points. Data are shown as the mean ± SEM (two-tailed Student's t-test); n = 6 mice per group. (C) Tumor weights in mice treated as in (A) were recorded. Data are shown as the mean ± S.E.M. of three independent experiments (two-tailed Student's t-test). (D) QRT-PCR for HCP5 expression in xenograft tumor tissues of shHCP5 or shCtrl group. Data are shown as the mean ± S.E.M. of three independent experiments (two-tailed Student's t-test). (E) shHCP5 or shCtrl A549 cells were intravenously injected into nude mice and lung tissues were isolated after eight weeks. Representative lung images showing metastases in the lung. Arrows indicate the metastatic nodules. (F) Microscopic images of lung tissue sections stained by hematoxylin and eosin. Left: Scale bar = 200 μm. Right: Scale bar = 50 μm. (G) The number of metastatic nodules in the lungs is shown as the mean ± S.E.M.; n=4 mice per group (two-tailed Student's t-test). *P< 0.05, **P < 0.01, ***P < 0.001.