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. 2019 Apr 13;9(9):2489–2504. doi: 10.7150/thno.31159

Figure 2.

Figure 2

In vitro binding of CL-MOF, GS5-NS and GS5-MOF nanoparticles to activated platelet-coated surfaces versus platelet-deficient surfaces under flow conditions. The cubic GS5-MOFs show enhanced binding and aggregation with activated platelets compared to their spherical counterparts. (A) Quantitative surface averaged fluorescence intensity data at different time points. (B) Representative fluorescence microscopy images at 45 min. The large red fluorescent “patch” areas indicate the strong binding of GS5-MOF nanoparticles with the activated platelet-coated surface. GS5-MOF nanoparticles showed specific binding to activated platelet-coated surfaces (filled squares in red) and minimal adhesion to nontargeted control BSA surfaces (filled squares in green), while CL-MOFs without GRGDS modification showed minimal binding to activated platelet-coated surfaces (filled squares in blue). At least 10 images for each condition at each time were acquired for analysis. Scale bar = 50 μm. Values are the mean ± SD (n = 10). *Denotes a significant difference compared with the CL-MOF control. *p < 0.05, ***p < 0.001. #Denotes a significant difference (p < 0.05) between cubic GS5-MOF and spherical GS5-NS groups.