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. Author manuscript; available in PMC: 2019 May 19.
Published in final edited form as: ACS Appl Mater Interfaces. 2018 Aug 9;10(33):27621–27630. doi: 10.1021/acsami.8b08005

Figure 4.

Figure 4.

Photodestruction of SKBR3 cancer cells in vitro. (a) Fluorescent images of SKBR3 cancer cells incubated with PBS (negative control), 176 μM free ICG (positive control), and NETs ([ICGNETs] ≈ 144 μM) for 3 h at 37°C and followed by 808 nm laser irradiation at I0 = 680 mW/cm2 for 15 min. Live cells were identified by the Calcein AM stain and falsely colored in green. Dead cells in response to laser irradiation were identified using the EthD-1 stain and falsely colored in red. (b) Percentage viability of SKBR3 cells as a function of incubation agent. (c) Percentage viability of SKBR3 cells as a function of [ICGNETs]. In panels (b) and (c), statistically significant differences are indicated as *p < 0.05 and ***p < 0.001 (n = 3 samples for each treatment).