Figure 3.
HCT116 cell growth over time based on flow conditions (30 cell initial count). a, Normalized HCT116 cell count over time for various flow conditions and theoretical model predictions. b, Normalized nutrient availability within microfluidic system over time for various flow conditions. Equilibrium point indicates when nutrient availability within the system can no longer satisfy the requirements of the entire cancer cell population. c, Representative fluorescence images of HCT116 tumor spheroid growth over the course of 120 hours under a flow rate of 3.4×104 μL/hr (scale bars are 60μm). Statistical significance indicated by “*” between flow based tests and static conditions and “**” between flow based tests at p≤0.05.