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The drug effect on HCT116 tumor spheroids grown within the bi-layer microfluidic system by APH assay. Drug effects of Paclitaxel (a), Doxorubicin (b), and Capecitabine (c) on spheroids cultures, with comparison to monolayer cell cultures performed in flat bottomed well plates. Dose-response curves plotted exponentially over drug concentration range of 1nM to 1μM for all three drugs. All data points shown were run in replicates of four (n=4) for each testing condition and drug.
