Figure. 5.

Representative prototype of high-throughput array microfluidic system allowing 16 therapeutic screening tests to be run simultaneously. a, 3D model of high-throughput array microfluidic well plate designed for use with high-throughput imaging and data collection systems. b, Prototype well plate system manufactured via 3D printing being used to test data collection from a fluorescent microscope. c, Prototype well plate system being tested with a plate reader to verify data collection. d, Normalized fluorescence intensity data of FITC dye flown through the prototype high-throughput array system. Fluorescence intensity was measured at various dye concentrations while passing through the device and compared to fluorescence intensities at the same concentrations in standard well plates. The data collected by both methods are in good agreement, indicating that the prototyped system functions well plate reader data collection. Data collection tests were run five times (n=5) to achieve statistical significance.