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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Free Radic Biol Med. 2019 Apr 13;137:37–45. doi: 10.1016/j.freeradbiomed.2019.04.013

Fig. 3.

Fig. 3.

Role of 1O2 in phosphorylation-activation of PI3K/Akt and disulfide-inactivation of PTEN in U87 cells. ALA-treated cells were either not irradiated (DC) or irradiated (1 J/cm2) in the presence of 3 mM L-His or 3 mM L-Ala. After the indicated post-hv incubation times, samples were recovered for Western blot analyses of (A) p-PI3K (p-Tyr199/458) and total pI3K; (B) p-Akt (p-Ser-473) and total Akt; and (C) PTEN. Reducing conditions were used in (A) and (B), and non-reducing conditions in (C). The PTEN blot also shows the effects of H2O2 treatment (0.25 mM for 2 h).