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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Free Radic Biol Med. 2019 Apr 13;137:37–45. doi: 10.1016/j.freeradbiomed.2019.04.013

Fig. 4.

Fig. 4.

Total p300 and phospho-p300 levels before and after PDT: effects of a PI3K inhibitor. After pre-incubation with ALA, U87 cells were irradiated as described in Fig. 1. After the indicated periods of post-PDT incubation, cell samples were analyzed for total protein concentration, then subjected to Western blot analysis, using an antibody for overall p300 (A) or an antibody for p-p-300 (p-Ser-1834) (B). Other cells were treated with the PI3K inhibitor LY294002 (20 μM) immediately after irradiation, then checked for p-p300 levels at the indicated times (C). Dark controls (DC) −/+ LY294002 were analyzed as well. Total protein load per lane: 100 μg (A, B, C). Each immunoblot is a representative one from three replicate experiments; in each case, band intensities relative to β-actin and normalized to DC are plotted as means ± SEM (n=3); *P<0.01 vs. DC (plot B).