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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Free Radic Biol Med. 2019 Apr 13;137:37–45. doi: 10.1016/j.freeradbiomed.2019.04.013

Fig. 5.

Fig. 5.

Upregulation of p65-acK310 after a PDT challenge and mitigation thereof by a PI3K inhibitor or p300 inhibitor. (A) ALA-treated U87 cells were irradiated (cf. Fig. 1) and at the indicated post-hv times, probed for acetylated lysine-310 levels by immunoblotting, using an antibody against p65-acK310. Similarly, sensitized cells were treated with (B) LY294002 (20 μM) or (C) C646 (25 μM) immediately after irradiation and analyzed for p65-acK310 levels by Western blotting at the indicated post-hv times, along with appropriate dark controls. Each blot is representative of three replicate experiments, and the accompanying plot shows normalized relative band intensities as means ± SEM (n=3); *P<0.05 vs. DC (plot A).