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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Free Radic Biol Med. 2019 Apr 13;137:37–45. doi: 10.1016/j.freeradbiomed.2019.04.013

Fig. 7.

Fig. 7.

Effects of PDT on Sirt1 versus Sirt2 levels in U87 cells. U87 cells were exposed to ALA/light or ALA alone (DC) under conditions defined in Fig. 1. After the indicated post-irradiation times or 24 h for DC, samples were recovered for immunoblotting of Sirt1 (A) and Sirt2 (B). In (A), a sample of human recombinant Sirt1 was run alongside as a standard. For each analyte, a representative Western blot for an experiment run in triplicate is shown, along with a plot of band intensities relative to β-actin and normalized to DC; means ± SEM (n=3); *P<0.01 vs. DC (plot A).