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. Author manuscript; available in PMC: 2019 May 19.
Published in final edited form as: Nat Biotechnol. 2018 Nov 19:10.1038/nbt.4297. doi: 10.1038/nbt.4297

Figure 3. Full chemical stabilization of cholesterol-conjugated hsiRNAs enables systemic delivery to placental labyrinth.

Figure 3.

a. Structure and chemical composition of fully chemically-stabilized cholesterol-conjugated siRNAs (hsiRNA). b. Distribution of Cy3-hsiRNAsFLT1-2283 (red) in placentas of mice following intravenous (IV) or subcutaneous (SC) injection. Nuclei were stained with DAPI (blue). Matched slides were stained with hematoxylin and eosin (HE). Scale bars, 1 mm (upper panels), 25 μm (lower panels). c. Distribution of Cy3-labeled hsiRNAsFLT1-2283 (red) in mouse fetus. Nuclei were stained with DAPI (blue). Representative image of two experiments in which the dam received an IV injection of Cy3-labeled hsiRNAsFLT1-2283. d-e. Bar graphs of hsiRNAsFLT1-2283 levels in indicated tissues of mice from b. Guide strand levels were measure using the PNA hybridization assay. (n=3, mean ± SD). TGC, Trophoblast Giant Cells; SpT, Spongio Trophoblast; L, Labyrinth; Jz, Junctional zone; D, Decidua. (****P<0.0001; one-way ANOVA).