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. 2019 Apr 23;16:637–649. doi: 10.1016/j.omtn.2019.04.015

Figure 1.

Figure 1

Constructions of Minimal CRISPRa and CRISPRi Transgenes into pAAV Vector Backbone

To achieve efficient packaging of dSaCas9 fusion proteins and its sgRNA in a single AAV vector, a truncated version of the mouse Mecp2 brain-specific promoter (235 bp, pMecp2); a minimal polyadenylation signal (48 bp, spA); and the smallest version of the transcriptional regulator domain, such as VP64 activator (156 bp), VP160 activator (390 bp), KRAB repressor (227 bp), and SID4X repressor (432 bp), were used.