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. 2019 Apr 23;16:637–649. doi: 10.1016/j.omtn.2019.04.015

Figure 4.

Figure 4

Modulation of Other Endogenous Genes in Mouse N2a and Human U87 Cells

(A) Modulation of endogenous Mycn gene in N2a cells. The locations of different sgRNA target sites designed to target the promoter (C10, C11, C12, and E13) and gene body (E14 and E15) are shown. Right panel shows the relative expression levels of Mycn in N2a cells. (B) Modulation of endogenous Nrf2 gene in N2a cells. The locations of different sgRNA target sites designed to target the promoter (C16, C17, and C18) and gene body (E19, E20 and E21) are shown. Right panel shows the relative expression levels of Nrf2 in N2a cells. (C) Modulation of endogenous Keap1 gene in N2a cells. The locations of different sgRNA target sites designed to target the promoter (C22, C23, and C24) and gene body (E25, E26, and E27) are shown. Right panel shows the relative expression levels of Keap1 in N2a cells. (D) Modulation of endogenous PDGFRA gene in U87 cells. The locations of different sgRNA target sites designed to target the promoter (C28, C29, and C30) and gene body (E31, E32, and E33) are shown. Right panel shows the relative expression levels of PDGFRA in U87 cells. Red arrows indicate the sense or antisense orientation of sgRNAs designed to recognize target DNA sequences. The statistical significance levels from control (wild-type N2a or U87) are indicated as *p < 0.05, **p < 0.01, and ***p < 0.001. All data are presented as mean ± SD (n = 3).