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. 2019 Apr;7(8):170. doi: 10.21037/atm.2019.03.40

Figure 2.

Figure 2

Caspase1 inhibitor Ac-YVAD-CMK alleviated S. aureus-induced pyroptosis both in vivo and in vitro. (A) mRNA levels of caspase1 and GSDMD were determined by quantitative real-time PCR with or without S. aureus and Ac-YVAD-CMK (0.5, 5, 50 µM) in MC3T3-E1 cell line; (B) western blot analysis of caspase1 and GSDMD in vitro; (D) western blot detection of caspase1 and GSDMD in mice. (n=6 in each group, 2 randomly selected samples in each group); (C,E) quantification of (B) and (D); *, P<0.05, compared to PBS; #, P<0.05, compared to S. aureus. PBS, phosphate-buffered saline.