Table 1.
Primer sequences for qPCR.
| Gene symbol | Gene name | Forward sequence (5′-3′) | Reverse sequence (5′-3′) |
|---|---|---|---|
| Human primers | |||
| ATG4D | Autophagy related 4D cysteine peptidase | CTCAACCCCGTGTATGTGC | TACAGTGAGTGTCGCGGTTT |
| ATG9B | Autophagy related 9B | GCTACTGGGACATCCAGGTG | AAGAGGCGGGACTGCAC |
| CAT | Catalase | ACTTTGAGGTCACACATGACATT | CTGAACCCGATTCTCCAGCA |
| FIS1 | Mitochondrial fission 1 protein | TGACATCCGTAAAGGCATCG | CTTCTCGTATTCCTTGAGCCG |
| GPX | Glutathione peroxidase 1 | CCAGTCGGTGTATGCCTTCTC | GAGGGACGCCACATTCTCG |
| HMOX1 | Heme oxygenase 1 | GCCAGCAACAAAGTGCAAG | GAGTGTAAGGACCCATCGGA |
| HPRT1 | Hypoxanthine phosphoribosyltransferase 1 | CCTGGCGTCGTGATTAGTGAT | AGACGTTCAGTCCTGTCCATAA |
| LAMP1 | Lysosomal-associated membrane protein 1 | ACGTTACAGCGTCCAGCTCAT | TCTTTGGAGCTCGCATTGG |
| MAP1LC3B | Microtubule associated protein 1 light chain 3 beta | GAGAAGACCTTCAAGCAGCG | TATCACCGGGATTTTGGTTG |
| MCOLN1 | Mucolipin 1 | TTGCTCTCTGCCAGCGGTACTA | GCAGTCAGTAACCACCATCGGA |
| MFN2 | Mitofusin 2 | ATGTGGCCCAACTCTAAGTG | CACAAACACATCAGCATCCAG |
| PPARGC1A | Peroxisome proliferator-activated receptor gamma, coactivator 1 alpha | AGCCTCTTTGCCCAGATCTT | CTGATTGGTCACTGCACCAC |
| PPARGC1B | Peroxisome proliferator-activated receptor gamma, coactivator 1 beta | CCACATCCTACCCAACATCAAG | CACAAGGCCGTTGACTTTTAGA |
| POLG | DNA polymerase gamma, catalytic subunit | GAAGGACATTCGTGAGAACTTCC | GTGGGGACACCTCTCCAAG |
| PPIA | Peptidylprolyl isomerase A | CAGACAAGGTCCCAAAGACAG | TTGCCATCCAACCACTCAGTC |
| PRC | PPARG related coactivator 1 (PPRC1) | GTGGTTGGGGAAGTCGAAG | TGACAAAGCCAGAATCACCC |
| SOD1 | Superoxide dismutase 1, soluble | AGGGCATCATCAATTTCGAGC | GCCCACCGTGTTTTCTGGA |
| SOD2 | Superoxide dismutase 2, mitochondrial | CAGACCTGCCTTACGACTATGG | CGTTCAGGTTGTTCACGTAGG |
| SIRT1 | Sirtuin 1 | TCAGTGTCATGGTTCCTTTGC | AATCTGCTCCTTTGCCACTCT |
| SIRT3 | Sirtuin 3 | TGGAAAGCCTAGTGGAGCTTCTGGG | TGGGGGCAGCCATCATCCTATTTGT |
| TFAM | Transcription factor a, mitochondrial | CCATATTTAAAGCTCAGAACCCAG | CTCCGCCCTATAAGCATCTTG |
| TP53 | Tumor protein p53 | TCAACAAGATGTTTTGCCAACTG | ATGTGCTGTGACTGCTTGTAGATG |
| TXN2 | Thioredoxin 2 | TGATGACCACACAGACCTCG | ATCCTTGATGCCCACAAACT |
| TWIST1 | Twist family BHLH transcription factor 1 | CGGAGAAGCTGAGCAAGATT | TGGAGGACCTGGTAGAGGAA |
| VIM | Vimentin | GAGAACTTTGCCGTTGAAGC | TCCAGCAGCTTCCTGTAGGT-3 |
| WIPI1 | WD repeat domain, phosphoinositide interacting 1 | CCTCCTGGATATTCCTGCAA | GCACAATCTCCCCTGAAGTC |
| ZEB1 | Zinc finger E-box binding homeobox 1 | AGTGATCCAGCCAAATGGAA | TTTTTGGGCGGTGTAGAATC |
| ZEB2 | Zinc finger E-box binding homeobox 2 | AACAAGCCAATCCCAGGAG | GTTGGCAATACCGTCATCCT |
| Mouse primers | |||
| Hprt1 | Hypoxanthine phosphoribosyltransferase 1 | TCAGTCAACGGGGGACATAAA | GGGGCTGTACTGCTTAACCAG |
| Ppargc1a | Peroxisome proliferator-activated receptor gamma, coactivator 1 alpha | AGCCGTGACCACTGACAACGAG | GTCGCATGGTTCTGAGTGCTAAG |
| Ppargc1b | Peroxisome proliferator-activated receptor gamma, coactivator 1 beta | CCCAGCGTCTGACGTGGACGAGC | CCTTCAGAGCGTCAGAGCTTGCTG |
| Ppia | Peptidylprolyl isomerase A | GAGCTGTTTGCAGACAAAGTTC | CCCTGGCACATGAATCCTGG |